Detection of the single nucleotide polymorphism (rs2227307) in the human interleukin 8 gene using a different PCR-RFLP assay

Detection of the single nucleotide polymorphism (rs2227307) in the human interleukin 8 gene using a different PCR-RFLP assay

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Interleukin 8 (IL-8) is chemokine that acts as a potent chemoattractant for neutrophils.Single nucleotide polymorphisms (SNPs) in the human IL8 gene have been investigated in many studies for disease association.We have developed a different PCR-RFLP (Polymerase Chain Reaction - Restriction Fragment of Length Polymorphism) assay for genotyping the SNP (rs2227307) in the IL8 gene.

This method was utilized in order to typing 147 white healthy Medical and Ambulatory supplies / Physical Therapy Brazilian individuals whose DNA were obtained from epithelial buccal cells and extracted with phenol:chlorophorm:isoamilic alcohol.Genomic DNA was amplified by PCR using a conventional thermal cycler; following the PCR products (573 bp) were submitted to the RFLP reactions.The RFLP products were analysed in a 4% agarose gel stained with ethidium bromide.

The genotype distribution observed in this study was consistent with the assumption of Hardy-Weinberg equilibrium and was similar (p=0.30) to those reported for other Dog Kennels white populations at the website of the National Center for Biotechnology Information (NCBI) SNP Database.Because the PCR-RFLP method presented here was efficient, low cost, reproducible and convenient for worldwide technology limited laboratories, should be useful for genotyping in case-control association or population genetic studies.